Biotypmg of Pasieurella multocida isolates isolated from chickens in Egypt by RAPD PCR

The incidence of P. multocida isolates isolated from 50 random samples collected from chicken suffering from respiratory manifestation was 48%, the random serological classification showed 4 isolates [A:5, A:8, A:9and D:2]. RAPD-PCR using 4 random primers separately was demonstrated to characterize, identify and determine the differences in the DNA sequence among the isolates. Pasteurella multocida is one of the most important Gram-negative bacteria. It is upper respiratory tract [or pharyngeal region] commensal of many animal species. However, under predisposing factors the organism becomes the etiological agent of a wide range of economically important diseases, such as fowl cholera in poultry, hemorrhagic septicemia in cattle and buffalo, atrophic rhinitis in swine and snuffles in rabbits

Effect of preoperative aspirin on blood lossand blood transfusion in patients undergoingcoronary artery bypass grafting: Impactof discontinuation prior to surgery

The aim of our study is to evaluate the impact of recent aspirin use before CABG on the post operative bleeding, blood transfusion and re-exploration. Retrospectively collected data from 67 patients who were operated in Sultan Qaboos University Hospital from 2008-2009 was included in this study. Patients were divided into two groups, [1] Aspirin stopped >7 days before surgery [34 patient] and [2] Aspirin received within 7 days before surgery [33 patients]. Platelet transfusion and length of hospital stay was more in patients where Aspirin stopped < 7 days [P value 0.03 and 0.002 respectively]. There was trend of increase in intraoperative bleeding and PRBC transfusion in the group who received Aspirin within seven days. Postoperative blood loss was higher in aspirin users than non aspirin users but not statistically significant. There was no difference between two groups regarding re-exploration or operative mortality. Patients who take aspirin within 7 days before surgery have more tendency to have post-operative bleeding and receiving blood products. They also have longer length of stay in the hospital. Our recommendation is to stop aspirin more than 7 days before surgery

Biochemical study of iron metabolism and some elements in beta-thalassaemic children with stunted growth

Forty beta-thalassemic children [20 splenectomized and 20 non- splenectomized] matched with twenty children free from any hematological abnormalities [control group] were studied. Both groups were subjected to full clinical examination complete hemogram, hemoglobin electrophoresis and were all assayed for serum iron, total iron binding capacity, transferrin saturation, non-transferrin plasma iron, serum ferretin, ceruloplasmin, serum zinc, copper and blood lead. The results showed elevation of serum iron, non transferrin plasma iron, serum ferretin, ceruloplasmin, serum copper and blood lead in beta-thalassemic patients more in the splenectomized group significant reduction in serum zinc level